Journal: Aging and Disease

Article Title: Microcirculation Dysfunction in Subacute Stroke: The Role of Delayed Capillary Pericyte Loss

doi: 10.14336/AD.2025.0197

Figure Lengend Snippet: Short-term effects of fasudil on microcirculation following tMCAO. (A) Representative two-photon image showing changes in capillary lumen diameter following tMCAO. Scale bar = 20μm. (B) Quantification and comparison of mean normalized capillary lumen diameter changes at pericytes locations. (C) Representative image showing changes in TCV following tMCAO. Scale bar = 50μm. (D) Quantification and comparison of TCV changes in saline and fasudil groups. (E) Representative two-photon image showing changes in visible pericytes following tMCAO. Scale bar = 50μm. (F) Quantification and comparison of the number of visible pericytes in saline and fasudil groups. (G) Representative image showing changes in capillary stalls following tMCAO. Scale bar = 20 µm. (H) Quantification and comparison of the mean number of capillary stalls in saline and fasudil groups. (I) Quantification and comparison of the mean number of capillary stalls at pericyte covered segment in saline and fasudil groups. In panels B, D, F, H, and I, two-tailed Student’s t-tests were used for statistical. N =6 per group. * p < 0.05. Data are presented as mean ± SD.

Article Snippet: Fasudil hydrochloride (MedChem Express, USA) was administered intraperitoneally at a dose of 10 mg/kg once daily for 14 days post-reperfusion.

Techniques: Comparison, Saline, Two Tailed Test

Journal: Aging and Disease

Article Title: Microcirculation Dysfunction in Subacute Stroke: The Role of Delayed Capillary Pericyte Loss

doi: 10.14336/AD.2025.0197

Figure Lengend Snippet: Effects of fasudil combined with Nec-1 on reducing pericyte necroptosis following tMCAO. (A-B) Representative immunofluorescence images and quantification of p-MLKL + /PDGFRβ + cells on day 7 post-reperfusion. Scale bar = 50μm. (C) Schematic illustration of the ischemic penumbra zone. The gray area represents the infarct core, and the white area indicates normal brain tissue. (D-F) Representative Western blot images and quantification of p-RIP1 expression normalized to RIP1, and p-MLKL expression normalized to MLKL, in cortical tissue from the ischemic penumbra zone. In panels B, E, and F, one-way ANOVA was used for statistical. N = 5 per group. * p < 0.05; ** p < 0.01; *** p < 0.001. Data are presented as mean ± SD.

Article Snippet: Fasudil hydrochloride (MedChem Express, USA) was administered intraperitoneally at a dose of 10 mg/kg once daily for 14 days post-reperfusion.

Techniques: Immunofluorescence, Western Blot, Expressing

Journal: Aging and Disease

Article Title: Microcirculation Dysfunction in Subacute Stroke: The Role of Delayed Capillary Pericyte Loss

doi: 10.14336/AD.2025.0197

Figure Lengend Snippet: Treatment with fasudil combined with Nec-1 improves capillary perfusion and pericyte survival during the subacute phase following tMCAO. (A) Representative image and quantification of visible pericytes among different treatment groups. Scale bar = 50μm. (B) Representative image and quantification of TCV among different treatment groups. Scale bar = 50μm. (C) Representative image and quantification of mean normalized capillary lumen diameter among different treatment groups. Scale bar = 20μm. (D) Representative image and quantification of capillary stalls among different treatment groups. Scale bar = 20μm. In panels A-D, one-way ANOVA was used for statistical. N = 6 per group. * indicate comparisons between the fasudil+Nec-1 and saline groups, and # indicate comparisons between the fasudil+Nec-1 and fasudil groups. * p < 0.05; ** p < 0.01. Data are presented as mean ± SD.

Article Snippet: Fasudil hydrochloride (MedChem Express, USA) was administered intraperitoneally at a dose of 10 mg/kg once daily for 14 days post-reperfusion.

Techniques: Saline

Journal: Aging and Disease

Article Title: Microcirculation Dysfunction in Subacute Stroke: The Role of Delayed Capillary Pericyte Loss

doi: 10.14336/AD.2025.0197

Figure Lengend Snippet: Therapeutic effects of fasudil combined with Nec-1 on cerebral injury following tMCAO. (A) Representative LSCI images of cerebral blood flow in different groups at baseline and at day 14 post-reperfusion. (B) Quantification of regional cerebral blood flow in the ischemic penumbra among different groups. (C) Representative TTC-stained brain slices showing infarct regions. (D) Quantification of cerebral infarct volume. (E) Representative brain slices showing Evans blue extravasation; blue areas indicate regions of dye leakage. (F) Quantification of Evans blue content in brain tissue. (G) Representative immunohistochemical images of IgG extravasation. Higher magnifications views of the boxed areas are shown below, highlighting IgG-positive regions. (H) Semi-quantitative analysis of IgG integrated optical density (IOD) to assess BBB integrity among different groups. (I) Modified neurological severity score (mNSS) assessed during ischemia, and on day 7 and 14 post-reperfusion. In panel B, # indicates comparisons between baseline and day 14 post-reperfusion within each group using paired-tests. In panels B, D, F, H, and I, * indicates comparisons among different treatment groups using one-way ANOVA. N = 6 per group. * p < 0.05, ** p < 0.01, *** p < 0.001. Data are presented as mean ± SD.

Article Snippet: Fasudil hydrochloride (MedChem Express, USA) was administered intraperitoneally at a dose of 10 mg/kg once daily for 14 days post-reperfusion.

Techniques: Staining, Immunohistochemical staining, Modification